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MENTOR

The journal of Underdog Mentoring. A peer-reviewed online journal for mentees to publish their work as a stepping stone to other publications in future.

Email contributions to info@underdogmentoring.com

 
Planting a Tree

The mission of the Mentor journal is to provide a home for scholarly articles of any field which are of high quality or have high potential. For many reasons, not all such articles find a suitable place in the peer-reviewed published literature. Rather than let these articles go unpublished, we publish them following light-touch peer review and editing. We allow submissions to follow their own formatting. Our approach fosters learning and confidence - providing a stepping stone for authors on their writing and publishing journey. To our knowledge we are the only journal which occupies the space between peer reviewed journals and non-peer reviewed publications.

 

“If you should be allowed to do it, then you should be allowed to do it for
money”: Continuing the prohibition of kidney markets

D Bettega

University of Sydney, Sydney, NSW 2006, Australia

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Date published: 30 June 2021

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The fundamental reason for establishing kidney markets is to address and overcome the ever-increasing organ shortage. I will be examining the argument for establishing kidney markets through Jaworsky and Brennan’s theory that ‘if you should be allowed to do it, then you should be allowed to do it for money’, and discuss why this theory should not be applied to support this argument. I believe that market trading in kidneys will inevitably result in the
commodification of people and I will validate this position by discussing three key arguments with reference to Beauchamp and Childress’ (1985) ethical framework of principlism. Principlism comprises four bioethical principles, justice (the equal distribution of goods), nonmaleficence (do no harm), respect for autonomy (self-governance and moral independence), and beneficence (do good)1. I will start by exploring the existing socioeconomic inequality
between poor organ sellers and wealthy organ buyers and how kidney markets will amplify this divide. Further, I will examine why kidney markets do not promote autonomous choices but merely legally allow the destitute to sell their organs due to their poor financial position. Finally, I will outline the conditions that commonly lead to end-stage renal disease, and subsequent kidney transplantation, and how the health and fitness markets can help combat the organ shortage by reducing the number of people in need of a kidney through better nutrition, fitness and health practices.

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3D BIOPRINTING: CURRENT SOCIAL, ETHICAL AND LEGAL ASPECTS

T Ruzmatov

University of Technology, Sydney, NSW 2007, Australia


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Date published: 29 June 2021

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Abstract


3D bioprinting has become one of the most anticipated technologies in the medical and scientific world. This technology could help overcome the lack of organ transplants, including for the heart. It may soon become possible to 3D bioprint immunocompatibile and patient-specific tissue on demand at the time of need. However, the rapid pace of development of this new frontier in regenerative medicine threatens to outstrip the ethical and regulatory considerations which accompany it. This paper explores these new ethico-legal concerns. Aspects analysed include exaggerated results, inflated public expectations, the influence of social media and how the current ethical climate may foster suboptimal standards of conduct by researchers or companies We discuss the socio-cultural and religious overlays influencing the field and current socio-ethical challenges. The technical progress is only as strong as the maturity of the bioethical and regulatory framework in which it flows within which it develops.

Keywords: 3D bioprinting, tissue engineering, biomedical cell product, regenerative medicine, cardiac regeneration, transplantation, ethics, legal regulation. regulation



Introduction

Every human life has value and saving life is one of the key objectives of medical science. This is perhaps especially true in transplantation which has recently achieved astonishing success since successful transplantation of not only human organs such as the kidneys, liver, lungs, etc., but even the transplantation of the heart. According to Australian Donation and Transplantation Activity Report, in Australia during 2017 the number of organ transplant recipients was 1,402 Australians.


However, a common global problem is a constantly increasing number of patients requiring organ transplants and the lack of donor organs for transplantation. Thus, according to the Global Observation on Donation and Transplantation, in 2017, 139,024 human organ transplant operations were performed worldwide, including 90,306 kidney transplants (36% of living donors), 32,348 liver transplants (19% of living donors), 7,881 heart transplants, 6,084 lung transplants, and 2,243 pancreas transplants. However, this large number of transplantations barely covers 10% of global needs. If these figures are extrapolated globally, the number of people in need of transplantation and those who die whilst waiting for the organ will continue to increase in the future.


These aspects predetermine the existence of a “black market” of human organs, the existence of criminal communities, including cross-border ones, involved in the illegal circulation of human organs and tissues. Demand creates supply, therefore, despite the almost universal ban on this circulation of human organs, the capacity of this black market on a global scale is billions of US dollars.


So, as an example, we can point to the Mastromarino case, considered in New York in 2008. Mastromarino was sentenced to 50 years for desecrating more than 1,800 corpses to be buried. This became possible due to the fact that he and his accomplices controlled several funeral homes. In fact, a criminal group organized a “supermarket” selling tissues and organs. Mastromarino even established Biomedical Tissue Services, which was a licensed company. In the course of the activities of this criminal group, more than ten thousand recipients received tissues and organs. These were taken without being tested for HIV, hepatitis, cancer, etc. As a result, a number of recipients were transfected with the corresponding diseases.


The book by Carla del Ponte on the participation of Kosovo Albanians in the killings of Serbs for the subsequent sale of human organs and tissues received even greater resonance. The leaders of the Kosovo Albanians, in particular Hashim Tachi, who was then the Prime Minister of Kosovo, were involved in this criminal story.


Perhaps the most apparent ethical argument against the commodification of human tissues and organs is that the creation of financial incentives for donation will undermine a central principle of bioethics — the free and informed consent of the donor. Financial incentives may even force impoverished people to give their organs to the more wealthy. The commercialisation of transplantology will lead to exploitation of poor people. As Goodwin argues, “selling organs will be tantamount to slavery, since weak, vulnerable social groups will turn into tool boxes, into boxes with usable spare parts devoid of humanity and individuality”. However, as she has also explored, it may be necessary to work with the objective fact that there is a market for human tissues and organs. Goodwin concludes that one must stop turning a blind eye to the obvious, and in order to reduce the black market, it is necessary to allow the legalised commercialization of organ turnover in order to recognize their civil objectivity and donor’s ability to remunerate their body posthumously.


The development of 3D bioprinting for cardiac regeneration is an attempt to develop a new medical therapy to address the deficiency of donor hearts. However, the rapid progress of 3D bioprinting threatens to outstrip its evidence base. Scientific and mainstream media hype may cloud the ability to reach reliable conclusions free from bias and exaggeration of results. The bold expectations of the research community are already reflected in some forecasts (Figure 1). It is notable that in the current year 2020, the forecast predicted the first implantable artificial heart and the image implies a close replica to the native human heart. There is a danger with infographics of this kind that they are not peer reviewed, not based on robust evidence and give the reader the impression of being more reliable than they are in reality. Perhaps future readers of this article in 2040 can attest to the accuracy of the projections at the time but this example highlights the problem that the true scientific messages required for the research community and the public to make decisions may become adulterated.


As well as the wrong message going out, the reception of the message may be influenced by social, cultural and religious phenomena.

Private companies exaggerating results and inflated dissemination by social media

Some commercial companies try to present their research using definitely bloated results. For example, the company (USA) presented a patient-specific technology for treatment of the end-stage heart failure. The company argues that a fully functioning heart will be created through 3D bioprinting using the patient’s own cells. On the company’s website there are many foggy information; furthermore, company declares “With BIOLIFE4D, a patient-specific, fully functioning heart will be created through 3D bioprinting and the patient’s own cells, eliminating the challenges of organ rejection and long donor waiting lists that plague existing organ transplant methods”. However, even last precise studies show that nowadays humanity does not have proper tools to create full and proper functional heart organ. But with a closer look we can find that the company can provide only so-called “Mini-hearts” or “Cardiac Muscle Patches”. The former ones lately show promising results on swines. Despite the loud statements on the main page of the site, BIOLIFE4D company’s scientists in their publication adhere to rather modest opinion. Moreover, the authors rightly emphasized that there are as technological as well regulatory challenges in this field, which attributed to the lack of large-scale commercial success in 3D bioprinting field.


Lack of actual and objective information about 3D bioprinting technology for the general public leads to situation when mass media inflates the findings of scientific papers. For example, in the Forbes Magazine the above mentioned company is presented in a much better light. Forbes published news about this company with title “BIOLIFE4D Just 3D Printed A Human “Mini-Heart”. Another vivid example of not quite truthful fact is published in a near-scientific mass media – Genetic Engineering & Biotechnology News Magazine. In April 2019, an article “First 3D Engineered Vascularized Human Heart Is Bioprinted” was published. Authors of this paper refer to the report from Noor et al (2019). From the title of the article one can conclude that scientists created a ready heart for transplantation, which of course is not so. In the original report, Noor et al discuss the potential of their approach for engineering personalised tissue and organ replacement in the future. Such exaggerated reports overestimate expectations of a particular technology, and when general people find out that this was not entirely true this could negatively impact research funding. Insufficient evidence and limitation of studies for reliable conclusion


Apart from exaggerated and inflated reports, there is another problem in proper development of 3D bioprinting technology, which relates to lack of robust evidence. First of all, it concerns the requirement to follow the guidelines and checklists for scientific publications. These simple steps can significantly enhance and improve scientific reports. Nowadays, there are two main guidelines in the animal research area: The ARRIVE Guideline and PREPARE Guideline. Furthermore, scientists have at their disposal the “Gold Standard Publication Checklist” that allows them to verify the quality of the article before submitting it to an editor. If we look at how many recent high-impact articles meet the criteria of these guidelines, we find that many papers do not follow the basic standards for good science. Some authors use an outdated guideline, do not indicate the year and reference for guideline, and some do not indicate that they followed any guideline at all (Table 1).

Continuing the topic of research limitations, we must highlight the additional problem of insufficient or lack of clearly visible results for qualitative reports. For instance, Noor et al (2019) argue that they produced fully vascularised and perfusable cardiac patches. Undoubtedly, the authors did a great job; however, in reality these patches are with just pipes inside, which 3D bioprinter printed according to its software (Figure 2).


Furthermore, authors did not provide the experiments on in vivo hearts but only implantation of the engineered patches between two layers of rat omentum. Which definitely cannot provide clear evidence of functioning of the vascularised patch and represents serious limitations to the study. In the concluding section of this paper, authors emphasise that long‐term in vitro studies and in vivo implantation experiments in animal models should be conducted in order to adequately evaluate the fate and therapeutic value of the printed tissues.


Zhang et al (2016) argue that they created "vascularisation" in their tissue patches. However, these structures are not perfusable and can demonstrate only “simulation results of flow velocity and oxygen distribution”. Furthermore, authors ignore the use of correct comparison tools. Many high impact studies in the 3D bioprinting field do not have the correct control groups.


As for animal experiments, scientists from this group did not show any in vivo experiments. Nevertheless, the authors state that such a technique could be translated to human cardiomyocytes derived from induced pluripotent stem cells to construct endothelialized human myocardium. However, this is not possible without extensive animal testing. Authors agree that further research should be done. It is expected that more advanced technologies are required to precisely print small diameter blood vessels within thick vascularised human heart constructs.

Ethical, social and religious perspectives

Any new technology needs to be accepted and supported by general society in order to reaching real success. The general public can influence on technology through socio-cultural and religious aspects. Under certain circumstances, some technologies that seem useful from the scientists’ point of view, still face difficulties in their development and dissemination. For instance, reproductive cloning is not accepted by the general public and therapeutic cell cloning is prohibited in France, Germany, Spain, Italy, Austria, Ireland, Israel, Sweden, Belgium, India, Canada and Australia but authorised in the UK, Denmark, Japan, the Netherlands and Korea . Furthermore, it is important to say that, despite their geographical proximity, the rules are very different. For instance, while it is authorised in Great Britain, it is prohibited in Ireland, authorised in Denmark but prohibited in Sweden.


Regarding the attitude of religion towards bioprinting research, we can say that it depends on many factors and types of religion. Different religions hold different views about a particular technology where human or animal cells are used. For example, the Catholic Church is completely opposed to research on human embryonic stem cells. Because the Church opposes deliberately destroying innocent human life at any stage, for research or any other purpose, it has a deontological morale opposition to all embryonic stem cell research. However, when scientists proposed avenues for possibly obtaining embryonic stem cells or their pluripotent equivalent without creating or harming embryos…?then what happened?. Moreover, Catholic leaders were among the first to welcome this idea. This attitude points to open opportunities for 3D bioprinting from Catholic point of view.


Islam, on the other hand, advocates research on embryonic stem cells as long as they benefit society with the least harm to embryos — the sources of stem cells. One of the teachings of the Qur’an says: “And whoever saves one - it is as if he had saved mankind entirely” (5:32). Separately it is necessary to say about the attitude to the ultimate goal of these studies. In case of disease, Islamic religion points people to seek proper treatment and to take it when possible and refers to hadith “Seek medical treatment, for truly Allah did not send down a disease without sending down a cure for it". However, we should take into account that many Islamic patients cannot accept the use of porcine tissue. Therefore, we can conclude with some reservations, that 3D bioprinting is permissible in that religion.

3D bioprinting adherents must take into account all aspects of human society; otherwise, the technology can meet insurmountable resistance. However, it is worth to say that any rejection of something new can drastically change with accumulation of personal experience, which includes, of course, the free acquisition of knowledge about capabilities of a particular technology. That is why any new technology must be explained in simple and understandable language so that it affects personal experience, motivation, emotions and leads to an objective intentional positive attitude of all sections of society without exception.

Fortunately, 3D bioprinting does not face fierce resistance in modern society as a whole. The social challenges around 3D bioprinting have received little commentary despite the schism between social promise and progress in technological terms. However, as scientists working towards the success of bioprinting, we have to maintain a clear understanding of this technology for all people.

Taking into account that our society is developing and perspectives of people are dynamic and what may not be acceptable today may be accepted after a few years from now. Anyway, we want to believe that more and more people will understand the need for this kind of research.

Legislation and regulation in different countries

As mentioned above, our society as a whole is not opposed to bioengineering in terms of socio-cultural and religious aspects; however, existing legal standards, regulatory processes and diversities can potentially limit the development of bioprinting technology. At the moment there are few regulations for bioprinting applied to research purposes. However, bioprinted products used in clinical applications should satisfy The Food and Drug Administration (FDA) or The General Medical Council (GMC) regulatory oversight in the future. The bio-inks which are used in the process of bioprinting should be manufactured using a strict regulatory guideline. The implementation of Current Good Tissue Practice (CGTP) and Additional Requirements for Manufacturers of Human Cells, Tissues, and Cellular and Tissue-Based Products (HCT/Ps) by FDA in the year 2011 aims to prevent the introduction or transmission of potential communicable diseases. The Australian Code of Good Manufacturing Practice (cGMP) follows the same principles. According to these documents there is an assumption that the 3D bioprinted tissues will not be regulated as human organs for transplantation but will be more likely to be regulated as a drug, device or biological products.

Furthermore, there are other critical criteria such as control of the quality and process validation procedures can also be applied along with good manufacturing practices, the bioprinted tissues still create difficulties in regulatory approval due to increasing complexity of clinical research. As of now, there are no manufacturing standards for bioprinting process and this current lack of regulatory actions needs to be rectified prior to the utilisation of 3D bioprinting on a clinically relevant scale.

Final thoughts

Bioprinting of tissues is a result of multidisciplinary team work, which includes biologists, engineers, computer science specialists and medicine professionals. Undoubtedly, it requires a significant amount of financial and specialist resources. According to PubMed library, in the last 5 years the number of publications of articles related to 3D bioprinting increased at 10 times (Table 2).


Scientists have demonstrated ground-breaking research in tissue fabrication. However, in this area there are still many challenges, related not to specific technological but also ethical and legal aspects. Considering a successful development, the pathway from 3D bioprinting of tissues or organs to implantation into a human contemporary society has to elaborate special protocols involving fabrication techniques, surgical and postsurgical care; furthermore, this pathway should avoid unnecessary hype and unobjective reports. It can be possible with development of highly repeatable and straightforward technologies to print the tissues and organs in logical steps, from simple to complex.


A great care should be taken to ensure that the financial resources for bioprinting studies are properly allocated, in the best interests of the potential patients and taxpayers. On the other hand, researchers and scientists should not provide false reports to attract more funds. A global registry for the pre-clinical, clinical and long-term follow-up trials in the bioprinting area will help in better transparency. Increased transparency, disclosure of information, and discussion of uncertainties regarding outcomes with clinical trial participants will also help to improve development of the technology.


A scientific-oriented regulation of the bioprinting process should be developed. 3D bioprinted products require a comprehensive regulation to assure quality control in every step of the process. In addition, for the successful development of bioprinting, the ethical aspects of the technology still need to be taken into account. Nowadays the vast majority of the researches have been made on laboratory animals. Before we truly learn how to bioprint human organs ready for implantation, our society must develop acceptable ethical standards for this technology. This is necessary so that when 3D bioprinting is translated to clinical practice developed we do not meet barriers to the wide clinical induction of technology in terms of ethical, legal or regulatory challenges.  

 

THORACIC TRAUMA

C Roche[1] & W Mustafa[2]

1 University Hospital of Wales, UK

2 Baghdad College of Medicine, Iraq

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Date published: 27 June 2021

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INTRODUCTION TO THORACIC TRAUMA


The assessment and management of any trauma patient begins with the primary survey which is the initial assessment to look for life-threatening injuries. This starts with a systematic ABC (airway-breathing-circulation) approach. If a life-threatening problem is identified it is treated before moving on to the next step of the assessment (see Management of the Severely Injured patient), although in larger teams individual team members may be allocated to one system, for example an anaesthetist may take charge of the airway.


In thoracic trauma the clinician must be alert to breathing problems due to injuries to the respiratory system and circulation problems due to haemorrhage into the thoracic cavity or direct injury to the heart or great vessels. Other structures can be injured and knowledge of the anatomy will allow the clinician to anticipate and diagnose injuries, describe them to other clinicians and treat them safely.


CLINICAL SCENARIO: a young male in his twenties is brought in to the trauma room after a road traffic accident. The paramedics have applied C-spine immobilisation. Your registrar is busy and asks you to assess the patient. How would you begin your assessment?



ANATOMY OF THE THORAX


Important Surface Landmarks


Midclavicular line: an imaginary line running vertically from the middle of the clavicle. For emergency needle decompression of a tension pneumothorax the needle is inserted where the midclavicular line meets the second intercostal space.


Midaxillary line: an imaginary line running vertically between the anterior and posterior axillary folds. Chest drains are inserted anterior to this line.


Sternal angle (of Louis): the palpable junction where the manubrium meets the body of the sternum. This marks the location of the attachment of the 2nd rib to the sternum and is used reliably find the second intercostal space. An imaginary horizontal plane through the sternal angle passes through the T4/5 intervertebral disc, marking the inferior boundary of the superior mediastinum and the level of the bifurcation of the trachea as well as the commencement and termination of the arch of aorta.


Ribs


Ribs 1-7 (true ribs) are directly attached to the sternum via the costal cartilages of each rib forming sternocostal joints.


Ribs 8-10 (false ribs) are indirectly attached. They articulate via costal cartilages with the costal cartilage of rib 7.


Ribs 11-12 (floating ribs) are not attached to the sternum or costal cartilages. They protect abdominal organs such as the kidneys or spleen.


The Intercostal Space


There are 3 layers of muscles: external, internal and innermost. The main intercostal neurovascular bundles run between the internal and innermost intercostal muscles beneath their respective ribs in the costal grooves. From superior (most protected in the groove) to inferior (least protected) the vessels are ordered vein, artery, nerve (VAN). When inserting a chest drain in an intercostal space it is inserted just above the rib below to avoid damage to the intercostal nerve.


The Pleura


This is a fibro-elastic membrane lined by squamous epithelial cells. It consists of two portions which are continuous with each other:


1. Parietal pleura. This is attached to the chest wall, deep to the innermost intercostal muscles. It can be divided into four parts according to the surface it lines (costal, diaphragmatic, mediastinal and cervical). The costophrenic angle is the acute angle formed as the costal pleura becomes diaphragmatic pleura. In the upright position, fluid can accumulate here and be seen on a chest x-ray as blunting of the costophrenic angles.


2. Visceral pleura. This is intimately attached to the surface of the lung.


Between the parietal and visceral pleurae is a potential space which can be filled to create an actual space, for example with air (pneumothorax) or blood (haemothorax).


The Thoracic Inlet and Outlet


The major arterial blood supply and venous drainage of the head and neck, the trachea and oesophagus pass through the thoracic inlet (also called the superior thoracic aperture), which is a bony ring.


Its boundaries are the 1st thoracic vertebra posteriorly, the upper border of the manubrium sterni anteriorly and the 1st ribs with their costal cartilages curving laterally. Many important structures can be injured here, including the apices of the lungs which extend just above the first ribs.


The trachea passes through the thoracic inlet and descends to the carina at the 4th thoracic vertebral level, where it divides into the right and left main bronchi. The oesophagus passes to the posterior mediastinum behind the trachea and if perforated it can cause a severe mediastinitis.


Anatomists refer to the the thoracic outlet (inferior thoracic aperture) as the large space between the thoracic and abdominal cavities. Its borders are: the 12th thoracic vertebrae posteriorly, the xiphisternum and costal margins anteriorly and the 11th and 12th ribs laterally. The diaphragm (which is composed of two domes) covers the thoracic outlet and separates the thorax from the abdomen. It has three major openings for the vena cava (at the level of T8), the oesophagus (at T10) and the aorta (T12). If the diaphragm is injured, abdominal contents can enter via the thoracic outlet into the chest.


Clinicians commonly use the term ‘thoracic outlet’ more loosely to refer to the space beneath the clavicle where vessels and nerves exit the thorax to supply the upper limbs. This is because clinically, these can become compressed as they run under the clavicle (the so-called Thoracic Outlet Syndrome).


CHEST INJURIES AND THEIR MANAGEMENT


Injuries can be classified by mechanism as blunt or penetrating as these have different clinical implications or they can be classified by anatomical location. Some injuries may be detected in the primary survey for life-threatening problems. After the primary survey, a secondary survey of the whole body is conducted by a structured approach which aims to pick up all injuries, including those which are not life-threatening.